Nonoxidative Strategy for Monitoring Peroxynitrite Fluctuations in Immune Responses of Tumorigenesis

Phagocyte respiratory burst in immune responses generates enormous amounts of reactive oxygen species (ROS) to fulfill primary defense against neoplasia. However, the beneficial functions associated with ROS, especially potent oxidant/nucleophile peroxynitrite, an immunological process are still ambiguous. Herein, we report construction and biological assessment cyanine-based fluorescent biosensors, which based on a nonoxidative strategy for peroxynitrite detection. The established is composed nucleophilic substitution nanoaggregate formation initiated by peroxynitrite. proposed this study could maintain cellular oxidative stress critical detection preserve homeostasis cell metabolism. remarkable sensitivity, reaction selectivity, spectral photostability our biosensors enabled us visualize endogenous levels immune-stimulated phagocytes. With aid basal imaging acute peritonitis model, visualization level variations tumorigenesis was accomplished assisted biosensors. It envisioned that provides promising tool early tumor diagnosis evaluation suppression without disturbing ROS signaling transduction.